u6 promoter specific primer (Eurofins)
86
Structured Review
Eurofins
u6 promoter specific primer
U6 Promoter Specific Primer, supplied by Eurofins, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+specific+primer/gene+primer+pyogenes+pyolysin+specific+t/pm41564866-893-23-27
Average 86 stars, based on 1 article reviews
U6 Promoter Specific Primer, supplied by Eurofins, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+specific+primer/gene+primer+pyogenes+pyolysin+specific+t/pm41564866-893-23-27
Average 86 stars, based on 1 article reviews
u6 promoter specific primer - by Bioz Stars,
2026-09
86/100 stars
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Purification:Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance Article Snippet: .. E. coli were harvested and the plasmids were column purified (Qiagen) and sequences were verified via Sanger sequencing of the plasmid with a Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance. Article Snippet: Our procedure made use of primers encoding a gRNA with BbsI overhangs and an additional G for hU6 RNApolIII transcription (Forward: 5 ′ -CACCGNNNNNNNNNNNNNNNNNNN-3 ′ and Reverse: 5 ′ -AAACNNNNNNNNNNNN NNNNNNC-3 ′ ), annealed by boiling (100 ◦ C for 5 min) and slowly cooling to room temperature (0.1 ◦ C per second until reaching 25 ◦ C) before ligating duplexes with a BbsI entry vector (Addgene #67974 or a hygromycin-mAzami Green version) using BbsI-HF (NEB), T4 DNA ligase and buffer (NEB), 1× BSA (NEB) for 30× cutting (37 ◦ C for 5 min) and ligating (16 ◦ C for 10 min) cycles, before heat-shock transformation of Stable Competent E. coli (NEB - C3040I) and spreading on 100 μg/mL ampicillin agar plates overnight at 37 ◦ C. Colonies were picked and expanded in LB containing 100 μg/mL ampicillin before shaking at 30 ◦ C overnight. .. E. coli were harvested and the plasmids were column purified (Qiagen) and sequences were verified via Sanger sequencing of the plasmid with a Sequencing:Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance Article Snippet: .. E. coli were harvested and the plasmids were column purified (Qiagen) and sequences were verified via Sanger sequencing of the plasmid with a Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance. Article Snippet: Our procedure made use of primers encoding a gRNA with BbsI overhangs and an additional G for hU6 RNApolIII transcription (Forward: 5 ′ -CACCGNNNNNNNNNNNNNNNNNNN-3 ′ and Reverse: 5 ′ -AAACNNNNNNNNNNNN NNNNNNC-3 ′ ), annealed by boiling (100 ◦ C for 5 min) and slowly cooling to room temperature (0.1 ◦ C per second until reaching 25 ◦ C) before ligating duplexes with a BbsI entry vector (Addgene #67974 or a hygromycin-mAzami Green version) using BbsI-HF (NEB), T4 DNA ligase and buffer (NEB), 1× BSA (NEB) for 30× cutting (37 ◦ C for 5 min) and ligating (16 ◦ C for 10 min) cycles, before heat-shock transformation of Stable Competent E. coli (NEB - C3040I) and spreading on 100 μg/mL ampicillin agar plates overnight at 37 ◦ C. Colonies were picked and expanded in LB containing 100 μg/mL ampicillin before shaking at 30 ◦ C overnight. .. E. coli were harvested and the plasmids were column purified (Qiagen) and sequences were verified via Sanger sequencing of the plasmid with a Plasmid Preparation:Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance Article Snippet: .. E. coli were harvested and the plasmids were column purified (Qiagen) and sequences were verified via Sanger sequencing of the plasmid with a Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance. Article Snippet: Our procedure made use of primers encoding a gRNA with BbsI overhangs and an additional G for hU6 RNApolIII transcription (Forward: 5 ′ -CACCGNNNNNNNNNNNNNNNNNNN-3 ′ and Reverse: 5 ′ -AAACNNNNNNNNNNNN NNNNNNC-3 ′ ), annealed by boiling (100 ◦ C for 5 min) and slowly cooling to room temperature (0.1 ◦ C per second until reaching 25 ◦ C) before ligating duplexes with a BbsI entry vector (Addgene #67974 or a hygromycin-mAzami Green version) using BbsI-HF (NEB), T4 DNA ligase and buffer (NEB), 1× BSA (NEB) for 30× cutting (37 ◦ C for 5 min) and ligating (16 ◦ C for 10 min) cycles, before heat-shock transformation of Stable Competent E. coli (NEB - C3040I) and spreading on 100 μg/mL ampicillin agar plates overnight at 37 ◦ C. Colonies were picked and expanded in LB containing 100 μg/mL ampicillin before shaking at 30 ◦ C overnight. .. E. coli were harvested and the plasmids were column purified (Qiagen) and sequences were verified via Sanger sequencing of the plasmid with a |